- Sample Quality Checks
- takes a few days
- core staff will usually send an email with the results
- Scheduling a Run
- you may have to wait for a flow cell to be full to sequenced
- this time can vary widely depending on the type of run and our workload.
- Sequencing
- once the flow cell is scheduled here are the approximate times
- clustering - 0.5 days
- sequencing
- 50SR - 2 days
- 100SR - 4 days
- 50PE - 4 days
- 100PE - 11 to 14 days
- Fastq generation
- usually done the week day the run finishes
- Basic Analysis
- ChIP-Seq or HITS-CLIP alignments and peak calling
- takes 1 to 2 days
- RNA-Seq
- take about a week
Information on equipment, experimental techniques, and informatics for the IDOM/DRC Functional Genomics Core (FGC) and Next-Generation Sequencing Core (NGSC) for Perelman School of Medicine at the University of Pennsylvania.
The blog lists recent posts here, use the Search Box to find posts of interest, e.g., ChIP-Seq. Please post comments and questions!
Wednesday, October 24, 2012
FAQ How Are My Samples Doing?
We will be extending the website and our notification system to provide more information but for now, here's what to expect.
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