Illumina miSeq

Summary
The NGSC has ordered a miSeq sequencer. This is a smaller capacity than a hiSeq, but is a much faster machine.

Availability
The miSeq is available now.

Sequencing Capacity

• Each miSeq has one flowcell.
• The flowcell has 1 lane.
• Each lane can generate about 15 million clusters.
• Each cluster can be sequenced from one end, single read (SR), or both ends, paired-end (PE).
• Sequencing is normally done to a length between 50bp and 250bp.
• A 250bp PE run can generate 7.5 × 10⁶ clusters × 500 bp/cluster = 3.75 × 10⁹ bp

So the miSeq lane capacity is close to the total base pair output of a hiSeq lane, but finishes in a day. However, to reach this capacity the miSeq is sequencing fewer but longer reads.

Using the miSeq

For now the miSeq will be operated by the NGSC staff.

However, we anticipate that after training investigators will be able to use the machine on their own.