Pippin Prep

Summary

The NGSC uses the Pippin Prep to do size selection of libraries.

Using Sage Science’s Pippin Prep helps standardize library size selection and most importantly vastly increases yield over the normal gel extraction technique.

• The machine does not catch your mistakes, so carefully follow the directions and be aware of what you are doing. 
• CAUTION: some gels contain Ethidium Bromide, so wear gloves at all times.

We recommend using 2% EF (Ethidium Free) Agarose Gel Cassette (Sage Science CEF-2010 or Life Technologies 4472171) for size selecting ChIP-Seq and RNA-Seq libraries.

3% EF Agarose Gel Cassettes (Sage Science CEF3010) can be used for size selection of smRNA-Seq samples.

1.5% EF Agarose Gel Cassettes (Sage Science CEF1510) can be used for size selection of gDNA libraries.