Summary
Because a single lane of a modern sequencer may have a capacity that exceeds what is necessary for a sample, Illumina (and other manufacturers) offer ways to put more that one library in a lane. Illumina does this by using modified adapters that include a barcode in one end or both. The barcodes are sequenced in distinct phases of the sequencing procedure. The reads from the lane are put into groups during the data processing phase.
Limitations
Illumina offers recommendations for sets of barcodes to use when pooling small numbers of libraries. Failure to follow these recommendations may result in the failure of the barcode sequencing and thus an inability to separate the libraries.
Sample Drop Off
When you bring samples in for sequencing, make sure you know what the barcodes are so that we can properly associate sample info with the barcode.
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